Research technician

The Pirbright Institute

The Pirbright Institute delivers world-leading research to understand, predict, detect and respond to viral disease outbreaks. We study viruses of livestock that are endemic and exotic to the UK, including zoonotic viruses, by using the most advanced tools and technologies to understand host-pathogen interactions in animals and arthropod vectors. Our major strength in understanding the immune systems of livestock provides a platform to control current diseases and respond to emerging threats. This important research would not be possible without the proportionate use of animals, which are integral to the development of new disease control measures such as vaccines and diagnostics. We have an establishment-wide commitment to the 3Rs (replacement, reduction and refinement), high standards of animal welfare, a culture of care and openness.

Our Institute is made up of a dynamic and vibrant community of staff covering a diverse set of chosen fields, backgrounds and experience. We all play a crucial role in furthering the Institute’s aspirations, advancing the frontiers of science and knowledge through our excellent scientific research, knowledge sharing and contribution; with a real focus in improving the world in which we live. Our outlook is always balanced by our strong sense of purpose, values and behaviours and an unwavering commitment to a ‘one Institute’ approach.

Organisation position and purpose

This research technician position is available within the Immunogenetics group on a 6 month fixed term contract, there is potential for this position to be extended for the Bill and Melinda Gates Foundation-funded Pirbright Livestock Antibody Hub for a duration of 30 months. The Hub exploits the recent advances in our understanding of B-cell responses to better determine the protective immune response in livestock and humans. A key component of our work is to enable antibody discovery, manipulation and testing in cattle, pigs and poultry.

We are now recruiting a research technician to study the B cell response post-vaccination in cattle. This post will play a leading role in driving the use of state-of-the-art techniques and technologies to understand the B cell and antibody responses at molecular detail.

This will involve using spectral flow cytometry, antigen-specific ELISpot, and a set of molecular assays developed to analyse the antibody response along the timeline of vaccination such as PCR and library preparation for antibody sequencing by NGS. The successful applicant will join a large established team working on antibody responses in livestock that includes specific expertise in bioinformatics and recombinant antibody production.

The successful applicant will start by a 6-months project on Lassa virus-specific antibody discovery in cattle, and will further apply these techniques on a range of diverse projects on foot-and mouth disease, bovine tuberculosis, and/or tick-born diseases subject to securing additional funding. This will provide them with a unique opportunity to become an expert on cattle B cell and antibody response analysis, using a broad range of state-of-the art assays.

The post-holder will report to, and work closely with, the leading research scientist on this project. Additional supervision and training will be provided from other members of the Immunogenetics group, Pirbright Antibody Hub, and the Immunological Toolbox team.

Main duties and responsibilities:

Designing, Performing, and Analysing Experiments:

• Assess the B cell and antibody responses post vaccination in cattle using a range of assays developed in the group.
• Collaborate with the research team to design experiments aimed at generating and isolating, antigen-specific antibodies.
• Execute laboratory experiments following established protocols and procedures.
• Analyze experimental data using appropriate statistical and analytical tools to draw meaningful conclusions.

Laboratory Management Duties:

• Ensure smooth operation of the laboratory by maintaining equipment, ordering necessary supplies, and managing inventory.
• Adhere to laboratory safety protocols and maintain a clean and organized work environment.
• Assist in the training of new laboratory personnel as needed.
• Coordinate the shipment of samples to external collaborators or testing facilities as necessary.
• Maintain electronic and physical records in compliance with laboratory protocols and regulations.

Presentation at Laboratory Meetings:

• Prepare and deliver presentations on experimental findings and progress at regular lab meetings.
• Engage in discussions with team members to troubleshoot experimental challenges and brainstorm solutions.

Assistance with Other Laboratory Tasks:

• Provide support to other members of the research team as required, including assisting with their experiments and projects.
• Collaborate on interdisciplinary projects within the lab to leverage expertise and resources.

All employees shall actively participate in the Institute-wide quality assurance system in conjunction with the Institute’s Quality Manager. Employees are also expected to safeguard their own health and safety and security by following policies and all employees are responsible for the Health and Safety and security of staff under their management.

Personal specification

• Intellectually engaged and results driven with a clear ability to time manage between multiple tasks
• Excellent collaboration skills
• Adaptability to changing priorities
• Attention to detail is essential
• Able to problem solve and be creative in generating a solution whilst also able to perform the same activity in a consistent manner
• Ability to work independently and as part of a team

Specific requirements

Essential

• A MSc or equivalent in a relevant area of Biosciences.
• Demonstrated skills in designing assays, including the incorporation of appropriate controls to ensure experimental rigor.
• Proficiency in molecular biology techniques including RNA extraction, cDNA synthesis, PCR, ELISA.
• Experience in next-generation sequencing, including library preparation and quality control procedures.
• Ability to analyse and compile data from multiple experiments.

Desirable

• Ability to analyze and synthesize data from diverse experiments including flow cytometry, ELISpots, sequencing, and 10X genomics.
• Knowledge of spectral flow would be advantageous.

Due to the nature of the work carried out by Pirbright, all successful applicants will be required to go through a comprehensive screening process including reference and qualification checks, financial and security clearances.

What we offer

• Generous Group Personal Pension Scheme and life cover (subject to age and earnings)
• 25 days annual leave per annum, pro rata, plus 10.5 public and privilege days
• Buy and sell annual leave
• Option to carry over up to two weeks annual leave
• Pirbright gym with subsidised membership
• Pirbright social club
• Pirbright Lifestyle Hub (employee discount site)
• Cycle to work
• Subsidised staff restaurant
• Childcare allowance
• Free onsite parking
• Electric Car charging facilities